Autoclave Use - Princeton UniversityExplore further
2021-10-1u2002·u2002Autoclave Cycles. To be effective, the autoclave must reach and maintain a temperature of 121° C for at least 30 minutes by using saturated steam under at least 15 psi of pressure. Increased cycle time may be necessary depending upon the make-up and volume of the load. The rate of exhaust will depend upon the nature of the load.
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